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Plemental Fig. S2). In contrast, I54A 32 was degraded slightly faster than wild type at 37 and even faster at 42 (Fig. 1 and supplemental Fig. S2).JOURNAL OF BIOLOGICAL CHEMISTRYChaperone Binding toFIGURE 1. In vitro degradation of 32 by ClpYQ and FtsH proteases. 32 (2.4 g) was incubated with ClpQ (1.0 g) and ClpY (2.5 g), or 32 (1.0 g) was incubated with FtsH (2.0 g), in a reaction mixture (6