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2, A and B), these results clearly indicate that amino acid substitutions in Region 2.1 resulted in a reduced affinity for DnaJ specifically.VOLUME 287 ?NUMBER 23 ?JUNE 1,19278 JOURNAL OF BIOLOGICAL CHEMISTRYChaperone Binding toFIGURE 3. Pull-down assay for complex formed between DnaK-His or DnaJ-His and 32. A, 32 (4 M) was incubated with DnaK-His (2 M) at 30 for 30 min in the absence ( ) or in